Axenic Growth and Development of the Cellular Slime Mould, Dictyostelium discoideum
نویسندگان
چکیده
منابع مشابه
Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.
1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.
متن کاملMetabolism of the cellular slime mould Dictyostelium discoideum grown in axenic culture.
1. The DNA, RNA, protein and carbohydrate contents of myxamoebae of Dictyostelium discoideum strain Ax-2 were measured after growth on bacteria or in various axenic media. 2. Myxamoebae grown in the different axenic media have similar DNA, RNA and protein contents, but there are marked differences in the contents of glycogen and free sugars. The DNA and protein contents of myxamoebae grown on b...
متن کاملEffect of growth conditions on development of the cellular slime mould, Dictyostelium discoideum.
The effect of growth in the presence and absence of 86 m\i glucose on fruiting-body number and size, fruiting-body proportions and morphogenesis in the slime mould Dictyostelium discoideum, strain Ax-2, has been investigated. Cells grown in the absence of glucose (N.S. cells) formed 2-1 limes more fruiting bodies than glucose-grown cells allowed to differentiate under the same conditions and at...
متن کاملEffect of arsenic on cell growth of the cellular slime mould, Dictyostelium discoideum.
The growing D. discoideum cells were killed in a dose-dependent manner when exposed to 100 and 140 ppm of arsenic (As2O3) at mid-log phase for 20 min. Reduced plaque sizes and changed cell and colony morphologies were observed in the treated cells. Endocytotic functions (both phagocytosis and pinocytosis) were also inhibited in the treated cells. Arsenic treated cell showed a lower DNA and prot...
متن کاملControl of cellular differentiation by temperature in the cellular slime mould Dictyostelium discoideum.
The effects of low temperature on morphogenesis and cellular differentiation of Dictyostelium discoideum were examined. During incubation at 5 degrees C, the vegetative and preaggregation cells never developed, but cell masses at the aggregation or slug stage developed to form hemispherical, or dumbbell-shaped multicellular structures. By staining with FITC-antispore IgG, the structures formed ...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1970
ISSN: 0022-1287
DOI: 10.1099/00221287-60-2-283